The image shows a portrait of a woman from the chest up. Her hair is intricately styled with decorative elements on top. She has a serene expression with soft facial features, slightly arching eyebrows, and a direct gaze. She is dressed in elaborate clothing typical of the period, featuring a high lace ruff collar, richly embroidered fabric, and accessories like a detailed brooch and a string of beads or pearls. The background features a simple, textured circular frame with inscriptions identifying her as "ILLUSTRISSIMA ISABELLA AUSTRIACA." An ornate Latin inscription is present at the bottom of the image.